Laboratory Support On Site
Academia & Industry

Houston & Surroundings

anita@laboratorysos.com
Your Subtitle text
ABOUT ME

Anita Myer Chandler, Ph.D.

832/910-5874

 anita@laboratorysos.com

 

SUMMARY

  • Over 25 years of scientific experience with 2 years in clinical work and 5 years in industry.
  • Organized, detail oriented, motivated biologist with a broad base and strong academic background in the fundamentals of mammalian molecular biology. 
  • Extensive experience in designing and implementing methodologies to study cellular processes in cell lines and animal models. 
  • Exemplary managerial skills maintaining the function of several laboratories as well as strong supervisory skills in training technicians, research assistants and graduate students.
  • A highly motivated self-starter and excellent problem solver.

EXPERIENCE

Self – Employed                                                                             LaboratorySOS April 2008 – Present

  • Established Laboratory Support On Site (www.laboratorysos.com) April 2008.
  • Obtained approved vendor status with M. D. Anderson Cancer Center and Baylor College of Medicine.
  • Contracted work for a medical technology company and a start-up biotechnology company; the Genetically Engineered Mouse Facility and a research laboratory in the Department of Biochemistry and Molecular Biology at M. D. Anderson Cancer Center; and Yancy Life Transition Center (non-profit).
  • Generated data for an SBIR grant; set up laboratories to GLP-like standards, including writing SOPs; worked as a murine ES cell technician; taught molecular biology to laboratory personnel and students; and taught pre-kindergarten to second graders arts and crafts.
  • Obtained HUB certification from the State of Texas.
  • Obtained LLC status in August of 2008.  

Research Assistant/ Lab Manager                           Baylor College of Medicine, Sept. 2009 – July 2013

  • Maintained, tracked, and ordered laboratory supplies.
  • Carried out the set-up of the laboratory.
  • Validated bioinformatic data via qRT-PCR and Northern analysis.
  • Assessed mRNA and protein degradation kinetics.
  • Assisted in generation of PAS-Seq libraries.
  • Cloned several < 5 Kb inserts into luciferase vectors.
  • Constructed various murine gene targeting vectors and created knock-out/in ES cells.
  • Genotyped murine ES cells and mice via PCR and Southern analysis
  • Trained and supervised research assistants, rotation students and graduate students.
  • Maintained a mouse colony for a second laboratory at Baylor College of Medicine.
Scientific Research Coordinator                                                  ApoCell, Inc.  March 2008 – May 2008
  • Implemented system for project flow from writing work-scope proposals to execution in the lab.
  • Wrote proposals for prospective oncology clients.
  • Implemented internal billing system from receipt of specimens to accounting.

 Manager, Quality Assurance                                                        ApoCell, Inc.  September 2007 – January 2008

  • Obtained certificate of registration from CLIA.
  • Logged in receipt of specimens and supplies.
  • Designed and wrote SOPs for CLIA and GLP compliance.
  • Implemented procedures via training for CLIA and GLP compliance.
  • Coordinated communication for an international cancer clinical trail.
  • Developed packaging of kits for use in cancer clinical trials and CLIA testing.

 Sr. Research Scientist                                                                     Molecular Logix, June 2005 – September 2007

  • Contracted to work for Kardia Therapeutics to determine that cardiac progenitor cells can be isolated from human heart tissue.   This resulted in the award of a phase II SBIR/STTR for May 2007 – April 2010.
  • Set up a GLP (i.e. filings of certificates of analysis and MSDSs, chemical inventory, writing SOPs, etc.)  laboratory at Baylor College of Medicine.
  • Developed SOPs for the successful isolation and propagation of proliferating cells from human autopsy and transplant cardiac tissue.
  • Maintained all proliferating populations and generated growth curves.
  • Initially advised a postdoctoral fellow in regards to histology and FACS analysis for the molecular characterization of the putative stem cells.
  • Isolated RNA and supervised the qRT-PCR of samples.
  • Participated in meetings concerning the future of Kardia Therapeutics, i.e. patent issues, requesting collaboration with other scientists, progress reports to BCMT.
  • Suggested and conducted the first quarterly meeting.

 Lead/Senior Scientist                                                                     Wyle Laboratories, August 2004 - March 2005

  • Supported Directed Strategic Research on the Bioastronautics Contract at NASA’s Johnson Space Center.
  • Ensured baseline operational readiness for three cell science laboratories.
  • Control account manager for three accounts and alternate for three more.
  • Implemented and maintained ISO 9001:2000 standards and VPP star safety status in three laboratories.
  • Performed chemical and equipment inventories and updated appropriate databases.
  • Completed and edited weekly activity reports for 4 subtasks.
Instructor/ Laboratory Manager                                                     M.D. Anderson Cancer Center, 2002 - 2004
  • Supervised and trained a graduate student in the embryoid differentiation assay.
  • Performed HSV and adenoviral transduction of C2C12 cells.
  • Derived myoblast cell lines from murine embryonic skeletal muscle.
  • Identified protein domain that is necessary for myofiber formation using embryoid body differentiation.
  • Designed and implemented a study of the molecular pathogenesis of human uveal melanoma employing WAVE and microarray technology.
  • Designed co-immunoprecipitation studies using C2C12 cells.
  • Adapted real-time PCR to quantify gene expression in murine muscle and embryonic stem cells.

 Research Associate/ Laboratory Manager                                    M.D. Anderson Cancer Center, 1998 - 2001

  • Maintained, tracked, and ordered laboratory supplies.
  • Supervised and trained laboratory technicians (1), research assistants (2) and graduate students (2).
  • Updated regulatory paperwork.
  • Organized and supervised relocation of laboratory and personnel.
  • Demonstrated phenotypic difference between myogenin and MyoD using engineered embryonic stem cells in the embryoid body differentiation assay.
  • Adapted cardiac embryoid differentiation assay for skeletal muscle differentiation.
  • Maintained and bred mice for the generation of double heterozygotes and knock-outs.

Postdoctoral Fellow                                                                           M.D. Anderson Cancer Center, 1994 - 1998

  • Established myogenin’s phenotypic function is non-cell autonomous.
  • Analyzed chimeric mice for muscle phenotype.
  • Adapted enzyme assay in order to quantify amount of chimerism.
  • Established murine embryonic stem cell lines of various genotypes from blastocysts.
  • Maintained a congenic heterozygous mouse colony.

Graduate Student                                                                               Univ. of Mass. at Amherst, 1988 - 1994

  • Taught Pathogenic Bacteriology and Immunology Laboratories.
  • Demonstrated polyubiquitin expression is differentially expressed between heat shock and programmed cell death in M. sexta.
  • Identified allelic polymorphisms of polyubiquitin are related to number of ubiquitin molecules.
  • Developed method to transfect muscles of M. sexta at the pupal stage.

 Research Technician                                                                         Harvard School of Public Health, 1986 - 1988

  • Maintained B-cell lines.
  • Established stable transfected B-cell lines.
  • Fluorescent Activated Cell Sorter (FACS) analysis

 Medical Technologist                                                                        Albany Medical Center Hospital, 1984 - 1986

  • Performed stat lab and toxicology tests.
  • Reported results to physicians.

 EDUCATION

Continuing
ISO/IEC 1705 Assessor Orientation                                   American Association of Laboratory Accreditation, 2013
Safety Management and Leadership                                 National Management Association,
Houston, TX
, 2005
Earned Value Management System                                   Wyle Laboratories, Houston, TX, 2005Project Management                                                                           Wyle Laboratories, Houston, TX, 2004

Degrees
Ph.D. in Molecular and Cellular Biology                              University of Massachusetts at Amherst, 1994
M.S. in Molecular and Cellular Biology                                University of Massachusetts at Amherst, 1990
B.S. in Medical Technology/Biology                                    Albany Medical Ctr. Hospital and SUNY Albany, 1985


GRANTS AND AWARDS

2001 – 2003         Grant awarded for the “Identification of Myogenin’s Fusion Domains” by the University Cancer Foundation at the University of Texas, M. D. Anderson Cancer Center, $50,000.

1995 - 1998            National Research Service Award, National Institutes of Health, National Institute of Arthritis, Musculoskeletal and Skin Diseases.

MEMBERSHIPS AND CERTIFICATIONS

2004 – present                    Member, American Association for the Advancement of Science
2004 – 2005                         Member, American Society for Gravitational and Space Biology
2001 – 2004                         Member, Federation of American Societies for Experimental Biology
1991 - 2004                         Member, Society for Developmental Biology
1985 - 1999                         Certified Medical Technologist, American Society of Clinical Pathologists


SCIENTIFIC EDITING

 

Publications

Ridgway, L.D., Wetzel, M.D. and Marchetti D (2010) Hepranase Modulates Shh and Wnt3a in Human Medulloblastoma Cells. Exp and Ther Med Open Access Article DOI: 10.3892/etm.2010.189.

Ridgway, L.D., Wetzel, M.D. and Marchetti D (2010)  Modulation of GEF-H1 Induced Signaling by Heparanase in Brain Metastic Melanoma Cells. J Cell Biochem 111(5):1299-309 (Feature article).


Zhang, L., Sullivan, P., Suyama, J. and Marchetti, D. (2010)
Epidermal Growth Factor-Induced Heparanase Nucleolar Localization Augments DNA Topoisomerase I Activity in Brain Metastatic Breast Cancer.  Mol Cancer Res 8(2):278-90. 

Grant proposals

2010          Department of Defense Breast Cancer Idea Award, Funded, priority score of 1.2, highest of study section

Baylor College of Medicine Dan L. Duncan  Cancer Center Development Research Award, Funded

National Institute of Health R0-1, Funded

2011       Avon Foundation, Funded 

Cancer Prevention and Research Institute of Texas, Bridging the Gap: Early Translational Research Awards.  Letter of intent submitted to Baylor College of Medicine, Accepted


 

PUBLICATIONS

Myer, A., Mason, H.A., Smith, W., Brown, C., and Schwartz, L.M. (2009) Differential Control of Cell Death and Gene Expression During Two Distinct Phases of Hormonally – Regulated Muscle Death in the Tobacco Hawkmoth Manduca sexta.  J. Insect Physio. 55(4):314-320.

Knapp, J.R.,
Davie, J.K, Myer, A., Meadows, E., Olson, E.N. and Klein, W.H. (2006) Loss of Myogenin in Postnatal Life Leads to Normal Skeletal Muscle but Reduced Body Size. Development 133
(4):601-610.

 
Myer, A., Olson, E.N., and Klein, W.H. (2001) MyoD Cannot Compensate for the Absence of Myogenin During Skeletal Muscle Differentiation in Embryonic Stem Cells. Dev Biol 229:340-350.

Myer, A., Wagner, D.S., Vivian, J.L., Olson, E.N. and Klein, W.H. (1997) Wild-Type Myoblasts Rescue the Ability of Myogenin-Null Myoblasts to Fuse In Vivo.   Dev Biol 185(2):127-138 (Cover Photo).

Myer, A. and Schwartz, L.M. (1996) Allelic Variation of Polyubiquitin in the Hawkmoth, Manduca sexta, and its Regulation by Heat Shock and Programmed Cell Death.  Insect Biochem  & Molec Biol  26(10):1037-1046.

Myer, A.  Developmentally Programmed Cell Death of the Intersegmental Muscles of Manduca sexta:  Emphasis on Polyubiquitin Expression.  Ph.D. Dissertation, University of Massachusetts, Amherst, MA, 1994.

Schwartz, L.M., Myer, A., Kosz, L., Engelstein, M., and Maier, C. (1990)  Activation of Polyubiquitin Gene Expression During Developmentally Programmed Cell Death.  Neuron 5(4):411-419.

Nabavi, N., Ghogawala, Z., Myer, A., Griffith, I.J., Wade, W.F., Chen, Z.Z., McKean, D.J., and Glimcher, L.H. (1989)  Antigen Presentation Abrogated in Cells Expressing Truncated Ia Molecules.  J Immunol 142(5):1444-1447.

Polla, B.S., Ohara, J., Paul, W.E., Nabavi, N., Myer, A., Liou, H.-C., Shen, F.-W., Gillis, S., Bonventre, J.V., and Glimcher, L.H. (1988)  Differential Induction of Class II Gene Expression in Murine Pre-B-Cell Lines by B-Cell Stimulatory Factor-1 and by Antibodies to B-Cell Surface Antigens.  J Mol Cell Immunol 3(6):363-373.

Griffith, I.J., Ghogawala, Z., Nabavi, N., Golan, D.E., Myer, A., McKean, D.J., and Glimcher, L.H. (1988)  Cytoplasmic Domain Affects Membrane Expression and Function of an Ia Molecule.  Proc Natl Acad Sci USA 85(13):4847-4851.

Acknowledgments

Zhang, L., Sullivan, P., Suyama, J. and Marchetti, D. (2010) Epidermal Growth Factor-Induced Heparanase Nucleolar Localization Augments DNA Topoisomerase I Activity in Brain Metastatic Breast Cancer.  Mol Cancer Res 8(2):278-90.

Posters

“Preliminary Characterization of Human Cardiac Progenitor Cells”, Noseda, M., Myer-Chandler, A., Tsai, J., Konemann, S., Schneider, M.D. Foundation Leducq Transatlantic Network of Excellence For Cardiac Regeneration, 3rd Annual Meeting of Network Investigators & Site Visit by the Foundation, Houston, TX, January 2007.

Oral Presentations

“Molecular and Transgenic Approaches for Uveal Melanoma”, Advances in Oncology Institutional Grand Rounds, M. D. Anderson Cancer Center, Houston, TX, July 2005.

“The Molecular Pathogenesis of Uveal Melanoma”, The Melanoma and Skin Cancer Research Program Seminar Series, M. D. Anderson Cancer Center, Houston, TX, May 2004.

 “Myogenin & Myoblast Fusion”


         a.    NASA’s Johnson Space Center, Houston, TX, July 2004.

      b.    Fels Institute for Cancer Research & Molecular Biology, Philadelphia, PA, April 2004.

Departmental Seminars, M. D. Anderson Cancer Center, Houston, TX:

          
a. “Further Investigation into the Unique Functions of MyoD and Myogenin”, February 2001.

          b. “MyoD Cannot Compensate for the Absence of Myogenin During Skeletal Muscle Differentiation in Embryonic Stem Cells”, Nov. 1999.

 Abstract selected for formal presentation: Myer, A., Wagner, D.S., Olson, E.N., and Klein, W.H. “Myogenin-Null Myoblasts Fuse with Wild Type Myoblasts, In Vivo”, Society for Developmental Biology, Nashville, TN, 1996.

“Activation of Polyubiquitin Gene Expression During Developmentally Programmed Cell Death”, Workshop on Hormones and Brain Function, Breckenridge, CO, 1994.

Website Builder